Two-state vs. multistate protein unfolding studied by optical melting and hydrogen exchange.
نویسندگان
چکیده
A direct conflict between the stabilization free energy parameters of cytochrome c determined by optical methods and by hydrogen exchange (HX) is quantitatively explained when the partially folded intermediates seen by HX are taken into account. The results support the previous HX measurements of intermediate populations, show how intermediates can elude the standard melting analysis, and illustrate how they confuse the analysis when they are significantly populated within the melting transition region.
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ورودعنوان ژورنال:
- Protein science : a publication of the Protein Society
دوره 9 10 شماره
صفحات -
تاریخ انتشار 2000